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Publications

The most recent publications from the Department of Chemical Biology can be found here:

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2026

  1. Pelvic Organ Prolapse with an Emphasis on the Central Compartment: From Genetic Risk Factors and Biomarkers to Contemporary Sacropexy and Emerging Robotic Innovations
    M Pomorski, T Fuchs, A Kryza-Ottou, J Budny-Wińska, J Śliwa, A Pomorski
    Journal of Clinical Medicine 15(13), 4967
    doi: 10.3390/jcm15134967
  2. Single-molecule FRET enables the detection of site-specific α-synuclein serine phosphorylation
    MH Choi, M Filius, S Klomp, YWI Trinh, A Pomorski, C Joo
    Chem 103030
    doi: 10.1016/j.chempr.2026.103030
  3. Structural domain plasticity drives Zn(II) distribution and buffering in metallothionein-3
    MD Peris-Díaz, EHTM Ebberink, J Fiala, AJR Heck, A Krężel
    Chem. Commun 62, 8942-8946
    doi: 10.1039/D6CC00567E

2025

  1. Modification of human metallothioneins by garlic organosulfur compounds, allicin and ajoene: direct effect on zinc homeostasis with relevance to immune regulation
    K Mosna, A Orzeł, M Tracz, S Wu, A Krężel
    Biometals 38(5), 1513-1533
    doi: 10.1007/s10534-025-00716-3
  2. Zinc-mediated dynamics of CD4/CD8α co-receptors and Lck kinase: implications for zinc homeostasis, immune response, and biotechnological innovations
    A Kocyła, A Krężel
    Metallomics 17(6):mfaf018
    doi: 10.1093/mtomcs/mfaf018
  3. Innately fluorescent tetravalent cytotoxic conjugate tetraFHER2-vcMMAE engages aggregation-dependent endocytosis of HER2 for enhanced intracellular drug delivery
    A Porębska, A Chorążewska, K Ciura, A Pomorski, A Krężel, Ł Opaliński
    J Med Chem 68(13):13872-13886.
    doi: 10.1021/acs.jmedchem.5c00782
  4. Biarsenical-based fluorescent labeling of metallothioneins as a method for ultrasensitive quantification of poly-Cys targets
    A Pomorski, S Wu, M Tracz, A Orzeł, J Bezdekova, A Brambor, A Suszyńska, K Piekarowicz, M Vaculovičová, A Krężel
    Analytica Chimica Acta 1337, 343550
    doi:10.1016/j.aca.2024.343550
  5. Deciphering the safeguarding role of cysteine residues in p53 against H2O2-induced oxidation using high-resolution native mass spectrometry
    MD Peris-Díaz, A Krężel, P Barran
    Communications Chemistry 8 (1), 13
    doi:10.1038/s42004-024-01395-w
  6. Electron capture-induced charge reduction benefits the recording of ultralong transients in orbitrap-based individual-ion mass spectrometry
    MD Peris-Díaz, A Barendregt, TP Wörner, KL Fort, AA Makarov, E Deslignière, AJR. Heck
    Analytical Chemistry Journal 97(22):11653-11660
    doi:10.1021/acs.analchem.5c01000
  7. RNA-associated glycoconjugates highlight potential ambiguities in glycoRNA analysis
    S Kim, Z Li, Y Choi, K Janssen, JWH Langenbach, DJ van den Brink, C Büll, BS. Joshi, A Pomorski, V Raz, ME. Tanenbaum, P Miesen, C Joo
    Experimental & Molecular Medicine 57:2619–2628
    doi:10.1038/s12276-025-01585-z

2024

  1. An efficient and easily obtainable butelase variant for chemoenzymatic ligation and modification of peptides and proteins
    AK Singh, A Antonenko, A Kocyła, A Krężel
    Microbial Cell Factories 23 (1), 325
    doi:10.1186/s12934-024-02598-5
  2. Site-specific and fluorescently enhanced installation of post-translational protein modifications via bifunctional biarsenical linker
    A Antonenko, A Pomorski, AK Singh, K Kapczyńska, A Krężel
    ACS omega 9 (45), 45127-45137
    doi:10.1021/acsomega.4c05828
  3. Antioxidant-related enzymes and peptides as biomarkers of metallic nanoparticles (eco)toxicity in the aquatic environment
    T Do, S Vaculciakova, K Kluska, MD Peris-Díaz, J Priborsky, R Guran, A Krężel, V Adam, O Zitka
    Chemosphere 364, 142988
    doi:10.1016/j.chemosphere.2024.142988
  4. Combining native mass spectrometry and proteomics to differentiate and map the metalloform landscape in metallothioneins
    MD Peris-Díaz, A Orzeł, S Wu, K Mosna, PE Barran, A Krężel
    Journal of Proteome Research 23 (8), 3626-3637
    doi:10.1021/acs.jproteome.4c00271
  5. Joint forces of mass spectrometric techniques (ICP-MS and MALDI-TOF-MS) and fluorescence spectrometry in the study of platinum-based cytostatic drugs interactions with metallothionein MT2 and MT3
    K Pavelicova, T Do, M Vejvodova, T Vaculovic, K Nowak, M Matczuk, S Wu, A Krężel, V Adam, M Vaculovicova
    Talanta 274, 125920
    doi:10.1016/j.talanta.2024.125920
  6. Phytochelatins bind Zn(II) with micro-to picomolar affinities without the formation of binuclear complexes, exhibiting zinc buffering and muffling rather than storing functions
    M Łuczkowski, W Leszczynska, J Wątły, S Clemens, A Krężel
    Inorganic Chemistry 63 (24), 10915-10931
    doi:10.1021/acs.inorgchem.4c01707
  7. A combined biochemical and cellular approach reveals Zn2+-dependent hetero-and homodimeric CD4 and Lck assemblies in T cells
    AM Kocyła, A Czogalla, I Wessels, L Rink, A Krężel
    Structure 32 (3), 292-303. e7
    doi:10.1016/j.str.2023.11.013
  8. Investigation of metal ion binding biomolecules one molecule at a time
    M de la Torre, A Pomorski
    Frontiers in Chemistry 12, 1378447
    doi:10.3389/fchem.2024.1378447

2023

  1. Differentiated Zn(II) binding affinities in animal, plant, and bacterial metallothioneins define their zinc buffering capacity at physiological pZn
    K Mosna, K Jurczak, A Krężel
    Metallomics 15 (10), mfad061
    doi:10.1093/mtomcs/mfad061
  2. Interaction patterns of methoprene-tolerant and germ cell-expressed Drosophila JH receptors suggest significant differences in their functioning
    M Kolonko-Adamska, A Zawadzka-Kazimierczuk, P Bartosińska-Marzec, W Koźmiński, G Popowicz, A Krężel, A Ożyhar, B Greb-Markiewicz
    Frontiers in Molecular Biosciences 10, 1215550
    doi:10.3389/fmolb.2023.1215550
  3. Structural characterization of Cu(I)/Zn(II)-metallothionein-3 by ion mobility mass spectrometry and top-down mass spectrometry
    MD Peris-Díaz, S Wu, K Mosna, E Liggett, A Barkhanskiy, A Orzeł, P Barran, A Krężel
    Analytical Chemistry 95 (29), 10966-10974
    doi:10.1021/acs.analchem.3c00989
  4. Calcium-assisted sortase A cleavage of SUMOylated metallothionein constructs leads to high-yield production of human MT3
    AK Singh, A Krężel
    Microbial Cell Factories 22 (1), 125
    doi:10.1186/s12934-023-02134-x
  5. Nesfatin-3 possesses divalent metal ion binding properties, which remain hidden in the nucleobindin-2 precursor protein
    A Skorupska-Stasiak, D Bystranowska, JB Tran, A Krężel, A Ożyhar
    Cell Communication and Signaling 21 (1), 165
    doi:10.1186/s12964-023-01181-6
  6. The connection of α-and β-domains in mammalian metallothionein-2 differentiates Zn(II) binding affinities, affects folding, and determines zinc buffering properties
    AK Singh, A Pomorski, S Wu, MD Peris-Díaz, H Czepczyńska-Krężel, A Krężel
    Metallomics 15 (6), mfad029
    doi:10.1093/mtomcs/mfad029
  7. From methodological limitations to the function of metallothioneins-a guide to approaches for determining weak, moderate, and tight affinity zinc sites
    A Pomorski, A Drozd, A Kocyła, A Krężel
    Metallomics 15 (5), mfad027
    doi:10.1093/mtomcs/mfad027
  8. OaAEP1 ligase-assisted chemoenzymatic synthesis of full cysteine-rich metal-binding cyanobacterial metallothionein SmtA
    A Antonenko, AK Singh, K Mosna, A Krężel
    Bioconjugate Chemistry 34 (4), 719-727
    doi:10.1021/acs.bioconjchem.3c00037
  9. Zn(II) to Ag(I) swap in Rad50 zinc hook domain leads to interprotein complex disruption through the formation of highly stable Agx(Cys)y cores
    O Kerber, J Tran, A Misiaszek, A Chorążewska, W Bal, A Krężel
    Inorganic Chemistry 62 (10), 4076-4087
    doi:10.1021/acs.inorgchem.2c03767
  10. Ion mobility mass spectrometry and molecular dynamics simulations unravel the conformational stability of zinc metallothionein-2 species
    MD Peris-Díaz, A Barkhanskiy, E Liggett, P Barran, A Krężel
    Chemical Communications 59 (30), 4471-4474
    doi:10.1039/d2cc06559b

2022

  1. One-step sortase-mediated chemoenzymatic semisynthesis of deubiquitinase-resistant Ub-peptide conjugates
    AK Singh, S Murmu, A Krężel
    ACS omega 7 (50), 46693-46701
    doi:10.1021/acsomega.2c05652
  2. An overlooked hepcidin–cadmium connection
    D Płonka, MD Wiśniewska, MD Peris-Díaz, A Krężel, AM Bonna, W Bal
    International Journal of Molecular Sciences 23 (24), 15483
    doi:10.1021/acsomega.2c05652
  3. An extremely stable interprotein tetrahedral Hg(Cys)4 core forms in the zinc hook domain of Rad50 protein at physiological pH
    M Łuczkowski, M Padjasek, J Ba Tran, L Hemmingsen, O Kerber, J Habjanič, E Freisinger, A Krężel
    Chemistry–A European Journal 28 (66), e202202738
    doi:10.1002/chem.202202738
  4. Non-conserved amino acid residues modulate the thermodynamics of Zn(II) binding to classical ββα zinc finger domains
    K Kluska, A Chorążewska, MD Peris-Díaz, J Adamczyk, A Krężel
    International Journal of Molecular Sciences 23 (23), 14602
    doi:10.3390/ijms232314602
  5. Does one plus one always equal two? Structural differences among nesfatin-1, nesfatin-2 and their complex
    R Lenda, M Padjasek, A Krężel, A Ożyhar, D Bystranowska
    Cell Communication and Signaling 20 (1), 163
    doi.org/10.1186/s12964-022-00980-7
  6. Relations between structure and Zn(II) binding affinity shed light on the mechanisms of Rad50 hook domain functioning and its phosphorylation
    JB Tran, M Padjasek, A Krężel
    International Journal of Molecular Sciences 23 (19), 11140
    doi:10.3390/ijms231911140
  7. Structures of silver fingers and a pathway to their genotoxicity
    K Kluska, G Veronesi, A Deniaud, B Hajdu, B Gyurcsik, W Bal, A Krężel
    Angewandte Chemie 134 (12), e202116621
    doi:10.1002/anie.202116621
  8. Drug conjugation via maleimide–thiol chemistry does not affect targeting properties of cysteine-containing anti-FGFR1 peptibodies
    K Jendryczko, J Rzeszotko, MA Krzyscik, A Kocyła, J Szymczyk, J Otlewski, A Szlachcic
    Molecular Pharmaceutics 2022, 19, 5, 1422–1433
    doi:10.1021/acs.molpharmaceut.1c00946

2021

  1. The bioinorganic chemistry of mammalian metallothioneins
    A Krężel, W Maret
    Chemical reviews 121 (23), 14594-14648
    doi:10.1021/acs.chemrev.1c00371
  2. An integrated mass spectrometry and molecular dynamics simulations approach reveals the spatial organization impact of metal-binding sites on the stability of metal-depleted proteins
    MD Peris-Díaz, R Guran, C Domene, V de Los Rios, O Zitka, V Adam, A Krężel
    Journal of the American Chemical Society 143 (40), 16486–16501
    doi:10.1021/jacs.1c05495
  3. HP1021 is a redox switch protein identified in Helicobacter pylori
    P Szczepanowski, M Noszka, D Żyła-Uklejewicz, F Pikuła, M Nowaczyk-Cieszewska, A Krężęl, K Stingl, A Zawilak-Pawlik
    Nucleic Acids Research 49 (12), 6863–6879
    doi:10.1093/nar/gkab440
  4. Phytochelatins as a dynamic system for Cd(II) buffering from the micro- to femtomolar range
    J Wątły, M Łuczkowski, M Padjasek, A Krężel
    Inorganic Chemistry 60 (7), 4657–4675
    doi:10.1021/acs.inorgchem.0c03639
  5. A guide to good practice in chemometric methods for vibrational spectroscopy, electrochemistry, and hyphenated mass spectrometry
    MD Peris-Díaz, A Krężel
    TrAC Trends in Analytical Chemistry 135, 116157
    doi:10.1016/j.trac.2020.116157
  6. InterMetalDB: a database and browser of intermolecular metal binding sites in macromolecules with structural information
    JB Tran, A Krężel
    Journal of Proteome Research 20 (4), 1889–1901
    doi:10.1021/acs.jproteome.0c00906
  7. Direct interaction of ATP7B and LC3B proteins suggests a cooperative role of copper transportation and autophagy
    S Pantoom, A Pomorski, K Huth, C Hund, J Petters, A Krężel, A Hermann, J Lukas
    Cells 10, 3118
    doi:10.3390/cells10113118
  8. Nucleobindin-2 consists of two structural components: The Zn2+-sensitive N-terminal half, consisting of nesfatin-1 and -2, and the Ca2+-sensitive C-terminal half, consisting of nesfatin-3
    D Bystranowska, A Skorupska, K Sołtys, M Padjasek, A Krężel, A Żak, M Kaus-Drobek, M Taube, M Kozak, A Ożyhar
    Computational and Structural Biotechnology Journal 19, 4300–4318
    doi:10.1016/j.csbj.2021.07.036
  9. Galvanization of protein–protein interactions in a dynamic zinc interactome
    A Kocyła, JB Tran, A Krężel
    Trends in Biochemical Sciences 46 (1), 64–79
    doi:10.1016/j.tibs.2020.09.006

2020

  1. Zinc-mediated dynamics of CD4/CD8α co-receptors and Lck kinase: implications for zinc homeostasis, immune response, and biotechnological innovations
    A Kocyła, A Krężel
    Metallomics. 2025 Jun 3;17(6):mfaf018.
    doi: 10.1093/mtomcs/mfaf018.
  2. Mass spectrometry-based structural analysis of cysteine-rich metal-binding sites in proteins with MetaOdysseus R software
    MD Peris-Díaz, R Guran, O Zitka, V Adam, A Krężel
    Journal of proteome research 20 (1), 776-785
    doi:10.1021/acs.jproteome.0c00651
  3. Metal-and affinity-specific dual labeling of cysteine-rich proteins for identification of metal-binding sites
    MD Peris-Díaz, R Guran, O Zitka, V Adam, A Krężel
    Analytical chemistry 92 (19), 12950-12958
    doi:10.1021/acs.analchem.0c01604
  4. A chemometric-assisted voltammetric analysis of free and Zn(II)-loaded metallothionein-3 states
    MD Peris-Díaz, L Richtera, O Zitka, A Krężel, V Adam
    Bioelectrochemistry 134, 107501
    doi:10.1016/j.bioelechem.2020.107501
  5. The glutathione/metallothionein system challenges the design of efficient O2‐activating copper complexes
    A Santoro, JS Calvo, MD Peris‐Díaz, A Krężel, G Meloni, P Faller
    Angewandte Chemie International Edition 59 (20), 7830-7835
    doi:10.1002/anie.201916316
  6. Metal exchange in the interprotein ZnII‐binding site of the Rad50 hook domain: structural insights into CdII‐induced DNA‐repair inhibition
    M Padjasek, M Maciejczyk, M Nowakowski, O Kerber, M Pyrka, W Koźmiński, A Krężel
    Chemistry–A European Journal 26 (15), 3297-3313
    doi:10.1002/chem.201904942
  7. Structural zinc binding sites shaped for greater works: Structure-function relations in classical zinc finger, hook and clasp domains
    M Padjasek, A Kocyła, K Kluska, O Kerber, JB Tran, A Krężel
    Journal of Inorganic Biochemistry 204, 110955
    doi:10.1016/j.jinorgbio.2019.110955
  8. Biarsenical fluorescent probes for multifunctional site-specific modification of proteins applicable in life sciences: an overview and future outlook
    A Pomorski, A Krężel
    Metallomics 12 (8), 1179-1207
    doi:10.1039/d0mt00093k
  9. Formation of highly stable multinuclear AgnSn clusters in zinc fingers disrupts their structure and function
    K Kluska, MD Peris-Díaz, D Płonka, A Moysa, M Dadlez, A Deniaud, W Bal, A Krężel
    Chemical communications 56 (9), 1329-1332
    doi:10.1039/c9cc09418k
  10. Formation of highly stable multinuclear AgnSn clusters in zinc fingers disrupts their structure and function
    K Kluska, MD Peris-Díaz, D Płonka, A Moysa, M Dadlez, A Deniaud, W Bal, A Krężel
    Chemical communications 56 (9), 1329-1332
    doi:10.1039/c9cc09418k

2019

  1. Chemometrics-assisted optimization of liquid chromatography-quadrupole-time-of-flight mass spectrometry analysis for targeted metabolomics
    MD Peris-Díaz, O Rodak, SR Sweeney, A Krężel, E Sentandreu
    Talanta 199, 380-387
    doi: 10.1016/j.talanta.2019.02.075
  2. Structural domain plasticity Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensingZn(II) distribution and buffering in metallothionein-3
    T Vaneckova, L Vanickova, M Tvrdonova, A Pomorski, A Krężel, T Vaculovic, V Kanicky, M Vaculovicova, V Adam
    Talanta 198, 224-229
    doi: 10.1016/j.talanta.2019.01.089
  3. Reactivity of Cu(II)–, Zn(II)– and Fe(II)–thiosemicarbazone complexes with glutathione and metallothionein: from stability to dissociation to transmetallation
    A Santoro, B Vileno, Ò Palacios, MD Peris-Díaz, Gilles Riegel, C Gaiddon, A Krężel, P Faller
    Metallomics 11, 994–1004
    doi: 10.1039/c9mt00061e

2018

  1. Short-sweep capillary electrophoresis with a selective zinc fluorescence imaging reagent FluoZin-3 for determination of free and metalothionein-2a-bound Zn2+ ions
    L Nejdl, A Moravanska, K Smerkova, F Mravec, S Krizkova, A Pomorski, A Krężel, M Macka, V Adam, M Vaculovicova
    Analytica chimica acta 1017, 41-47
    doi: 10.1016/j.aca.2018.02.008
  2. Metal binding properties, stability and reactivity of zinc fingers
    K Kluska, J Adamczyk, A Krężel
    Coordination Chemistry Reviews 367, 18-64
    doi: 10.1016/j.ccr.2018.04.009
  3. Optimized allosteric inhibition of engineered protein tyrosine phosphatases with an expanded palette of biarsenical small molecules
    S Korntner, A Pomorski, A Krężel, AC Bishop
    Bioorganic & Medicinal Chemistry 26:2610-2620
    doi: 10.1016/j.bmc.2018.04.026
  4. Crosstalk of the structural and zinc buffering properties of mammalian metallothionein-2
    A Drozd, D Wojewska, MD Peris-Díaz, P Jakimowicz, A Krężel
    Metallomics 10: 595–613
    doi: 10.1039/c7mt00332c
  5. Metal binding properties of zinc fingers with a naturally altered metal binding site
    K Kluska, J Adamczyk, A Krężel
    Metallomics 10(2), 248-263
    doi: 10.1039/c7mt00256d
  6. Metal binding properties of zinc fingers with a naturally altered metal binding site
    K Kluska, J Adamczyk, A Krężel
    Metallomics 10(2), 595–613
    doi: 10.1039/c7mt00256d
  7. Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing
    T Vaneckova, L Vanickova, M Tvrdonova, A Pomorski, A Krężel, T Vaculovic, V Kanicky, M Vaculovicova, V Adam
    Talanta 198, 224–229
    doi: 10.1016/j.talanta.2019.01.089
  8. Zinc clasp-based reversible toolset for selective metal-mediated protein heterodimerization
    A Kocyła, A Krężel
    Chemical Communications 54, 13539-13542
    doi: 10.1039/C8CC06301J
  9. Cu transfer from amyloid-β4–16 to metallothionein-3: the role of the neurotransmitter glutamate and metallothionein-3 Zn(ii)-load states
    A Santoro, NE Wezynfeld, E Stefaniak, A Pomorski, D Płonka, A Krężel, W Bal, P Faller
    Chemical Communications 54, 12634-12637
    doi: 10.1039/C8CC06221H
  10. Interdependence of free zinc changes and protein complex assembly – insights into zinc signal regulation
    A Kocyła, J Adamczyk, A Krężel
    Metallomics 10(1), 120-131
    doi: 10.1039/C8CC06221H

2017

  1. Fluorescent probes for selective protein labeling in lysosomes: a case of α-galactosidase A
    C Bohl, A Pomorski, S Seemann, AM Knospe, C Zheng, A Krężel, A Rolfs, J Lukas
    The Faseb Journal 31, 5258-5267
    doi: 10.1096/fj.201700058RRRR
  2. Molar absorption coefficients and stability constants of Zincon metal complexes for determination of metal ions and bioinorganic applications
    A Kocyła, A Pomorski, A Krężel
    Journal of Inorganic Biochemistry, 176, 53-65
    doi: 10.1016/j.jinorgbio.2017.08.006
  3. The Functions of Metamorphic Metallothioneins in Zinc and Copper Metabolism
    A Krężel, W Maret
    International Journal of Molecular Sciences 18, 1237
    doi: 10.3390/ijms18061237
  4. Eukaryotic Rad50 functions as a rod-shaped dimer
    YB Park, M Hohl, M Padjasek, E Jeong, KS Jin, A Krężel, JHJ Petrini, Y Cho
    Nature Structural & Molecular Biology 24, 248–257
    doi: 10.1038/nsmb.3369

Projekt "Zintegrowany Program Rozwoju Uniwersytetu Wrocławskiego 2018-2022" współfinansowany ze środków Unii Europejskiej z Europejskiego Funduszu Społecznego